质粒类型：慢病毒载体；四环素调控载体

启动子：TRE3GS

载体大小：9227 bp

载体抗性：氨苄青霉素

筛选标记：嘌呤霉素（Puromycin）

载体拷贝数：高拷贝

备注：慢病毒载体pLVX-TetOne-Puro是集调控与应答功能于一体的四环素诱导载体。

The Lenti-X Tet-One Inducible Expression System (Puro) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-G pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing and non-dividing mammalian cells. The all-in-one pLVX-TetOne-Puro Vector expresses the Tet-On 3G transactivator from the constitutive human PGK promoter in the forward orientation and your gene of interest from the PTRE3GS promoter in the reverse orientation. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene. In addition, this vector contains a puromycin antibiotic selection marker. The kit also includes Lenti-X Packaging Single Shots (VSV-G), a complete fourth generation lentiviral packaging system, and a sample of Lenti-X GoStix.

pLVX-TetOne-Puro产品说明书.pdf

Lenti-X Tet-One Inducible Expression Systems User Manual.pdf

pLVX-TetOne-Puro质粒序列.txt